引物设计软件

软件类型：软件>行业软件
软件性质：破解软件
操作系统：Windows
应用平台：Winll
问题反馈： http://www.oligo.net/contact.html
网站链接： http://www.oligo.net/
软件概述：

OLIGO 是一个从序列文件中搜索和选择寡核苷酸的多功能程序，应用于聚合酶链反应(PCR),DNA测序，定点突变及各种各样的杂交研究。
OLIGO根据最近邻热力学值计算寡核苷酸的杂交温度和二级结构。 这个好工具也用于构建合成基因，在已合成的基因中发现适当的序列引物，发现和多元化一致的引物和探针， 甚至发现蛋白质潜在的限制位点。

OlIGO 产品特色：
1. 分析TM和内部稳定性，绘制溶解温度图和稳定性图；
2. 给出寡核苷酸的重要相关信息；
3. 显示正向引物、反向引物和当前的OLIGO里潜在的连接物。潜在的二聚物也可显示出来；
4. 分析显示在选中的正向引物或反向引物中基本的成份和溶解温度；
5. 精确地分析出生物信息软件中的错配位点；
6. 正向引物和反向引物选中后，能自动产生PCR实验条件和PCR产物信息。

OLIGO Primer Analysis Software is the essential tool for designing and analyzing sequencing and PCR primers, synthetic genes, and various kinds of probes including siRNA and molecular beacons.
Based on the most up-to date nearest neighbor thermodynamic data, Oligo’s search algorithms find optimal primers for PCR, including TaqMan, highly multiplexed, consensus or degenerate primers. Multiple file batch processing is possible. It is also an invaluable tool for site directed mutagenesis.
For each primer or primer pair, Oligo’s various analysis windows show a multitude of useful data, such as DNA and RNA secondary structure, dimer formation, false priming and homology, internal stability, composition and physical properties. With Oligo you can analyze open reading frames down to predicted molecular weight and pKa of proteins, and search for restriction enzyme sites, not only in DNA but also in reverse-translated proteins.
The first version of the software appeared on the market in 1989. It went through several modifications, and the last one, the change from version 6 to 7, was the most comprehensive. Oligo 7 can automatically select multiplex primers, process sequence files in batch modes, automatically design PCR primers to cover multiple DNA regions in just one search and automatically find primer/probe sets for real time PCR or finds nested primers sets. Oligo search protocols (scoring system) may be customized in detail, so you may optimize the results according to your specific needs.
Oligo runs on Macintosh and Windows and it may be downloaded from this site. Click on the Tutorials link above to see Oligo 7 general overview and the major windows screen designs.

代码

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